中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 生命有机化学国家重点实验室  > 期刊论文
学科主题: 生命有机化学
题名: Purification and characterization of trichomaglin -A novel ribosome-inactivating protein with abortifacient activity
其他题名: 一种具有印产活性的新的核糖体失活蛋白-马干铃蛋白的纯化和表征
作者: Chen R(陈荣) ; Xu YZ(徐永镇) ; Wu J(邬键) ; Jin SW(金善炜) ; Liu YW(刘夷望) ; Xia ZX(夏宗芗)
刊名: Biochem. Mol. Biol. Int.
发表日期: 1999-01-01
卷: 47, 期:2, 页:185-193
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室; 上海药物所
英文摘要: Trichomaglin, a novel ribosome-inactivating protein, has been isolated from root tuber of a plant maganlin (Trichosanthes Lepiniate, Cucurbitacear). The isolation and purification procedure included ammonium sulfate precipitation, sephadex G-75 chromatography and CM-sephadex C-50 chromatography. The protein was identified to be homogeneous by SDS-PAGE and FPLC analysis. Its molecular weight is 24,673 dalton and isoelecttric point is 5.8, determined by electrospray ionization mass spectroscopy and isoelectric focusing gel electrophoresis respectively. Trichomaglin can inhibit protein synthesis in rabbit reticulocyte lysatewith ID=50 of 10.1 Nm. When rat ribosome was incubated with trichomaglin, a diagnostic RNA fragment appeared on polyacrylamide gel after ribosomal RNAs were treated with acidic aniline. It was concluded that trichomaglin is an RNA N-glycosidase. In addition, it has been verified to be an abortifacient protein.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23660
Appears in Collections:生命有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
990027.pdf(485KB)----开放获取--View Download

Recommended Citation:
Chen R,Xu YZ,Wu J,et al. Purification and characterization of trichomaglin -A novel ribosome-inactivating protein with abortifacient activity[J]. Biochem. Mol. Biol. Int.,1999,47(2):185-193.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[陈荣]'s Articles
[徐永镇]'s Articles
[邬键]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[陈荣]‘s Articles
[徐永镇]‘s Articles
[邬键]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 990027.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace