中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 高分子材料研究室  > 期刊论文
学科主题: 高分子化学
题名: Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake
其他题名: 带有疏水性芘的新型荧光两亲分子与质粒DNA的相互作用: 结合力、聚集以及细胞内吞
作者: Sheng RL(盛瑞隆) ; Luo T(罗挺) ; Zhu YD(朱英丹) ; Li H(李慧) ; Cao AM(曹阿民)
通讯作者: 曹阿民
刊名: Macromol. Biosci.
发表日期: 2010
卷: 10, 期:8, 页:974-982
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所
英文摘要: In this work, we prepared a novel series of cationic amphiphiles denoted as the Py-cations (Py-Gly, Py-Ala, Py-Cap, Py-G(1)-Lys and Py-G(2)-Lys) bearing fluorescent pyrene and various hydrocarbon linkers between the pyrene hydrophobe and cationic block. Employing these new cationic amphiphiles with pyrene as the fluorescent probe, the interactions between these Py-cations and plasmid DNA (pDNA) in distilled water and 0.1 M PBS buffer solution have been explored by means of UV-vis and fluorescent spectrometers, and ethidium bromide dye displacement and agarose-gel retardant assays were also implemented to evaluate their pDNA binding affinities in aqueous solution. Furthermore, the average sizes and morphologies of self-assembled Py-cation/pDNA lipoplex aggregates were examined by dynamic laser light scattering (DLS) and atomic force microscopy (AFM). It was found that these fluorescent cationic amphiphiles showed blue fluorescence emission of pyrene probe at lambda = 340 nm in distilled water while their interactions with pDNA led to new strong green emission at lambda = 490 nm, and this may be due to the stacking of pyrene and new formation of excimers via the rigid pDNA templated self-assembly. It was also revealed that the binding between new Py-cations and pDNA in aqueous solution was strongly influenced by the Py-cation hydrophobicity, charges of the cation and the presence of electrolytes. With respect to the Py-cation/pDNA aggregate morphologies, very interesting 1-D hybrid nanofibers were predominantly observed by AFM for the Py-Cap/pDNA aggregates. In addition, utilizing a COS-7 cell-line, in-vitro cellular uptakes of new cationic amphiphiles with pyrene probe were studied and visualized by fluorescent microscopy. As a result, this may provide a new approach to investigate the interactions between synthetic cationic lipids and nucleic acids, and pave an alternative clue to design new organic gene delivery carriers.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000281387900017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/22948
Appears in Collections:高分子材料研究室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2010117.pdf(572KB)----开放获取--View Download

Recommended Citation:
Sheng RL,Luo T,Zhu YD,et al. Interactions of New Synthesized Fluorescent Cationic Amphiphiles Bearing Pyrene Hydrophobe with Plasmid DNA: Binding Affinities, Aggregation and Intracellular Uptake[J]. Macromol. Biosci.,2010,10(8):974-982.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[盛瑞隆]'s Articles
[罗挺]'s Articles
[朱英丹]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[盛瑞隆]‘s Articles
[罗挺]‘s Articles
[朱英丹]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2010117.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace