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学科主题: 高分子化学
题名: Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification
其他题名: 聚乳酸-树枝状聚赖氨酸两亲基因载体的研究:赖氨酸代数的作用以及端基修饰增强转基因效率
作者: Li Y(李洋) ; Zhu YD(朱英丹) ; Xia KJ(夏可嘉) ; Sheng RL(盛瑞隆) ; Jia L(贾林) ; Hou XD(侯小东) ; Xu YH(徐宇虹) ; Cao AM(曹阿民)
通讯作者: 徐宇虹 ; 曹阿民
刊名: Biomacromolecules
发表日期: 2009
卷: 10, 期:8, 页:2284-2293
收录类别: SCI
部门归属: 上海有机所; 上海交通大学
英文摘要: As an effort to prepare new efficient gene delivery vectors, we have recently developed and reported an amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine) D-2-PLLA-D-2 with two-generation PLL dendrons and a PLLA block. In this work, we continued to explore the roles of dendritic PLL generation in DNA binding and intracellular delivery of gene, and a new series of amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine)s D-n-PLLA-D-n (n = 3-5) were synthesized and were structurally characterized. Furthermore, plasmid DNA binding affinity for these cationic amphiphiles was examined by agarose gel electrophoresis and fluorescence titration assay in pure water and PBS buffer solution containing 150 mM NaCl (pH = 7.4), respectively. By dynamic light scattering (DLS) and transmission electronic microscopy (TEM), the interaction and complexation in between were investigated, concerning the DNA/vector polyplex particle morphologies and zeta potentials. Utilizing a human hepatocellular carcinoma cell-line SMMC-7721, cell toxicity, and gene transfection in vitro were explored. To further improve transgene efficiency for these synthetic cationic amphiphiles as gene delivery vectors, new structural DEn-PLLA-DEn (n = 2-3) were prepared through an amino termini modification of the D-n-PLLA-D-n (n = 2-3) with less toxic 4,7,10,13-tetraazatridecanoic acids, and gene transfection with these DEn-PLLA-DEn (n = 2-3) was examined with an alternative human gastric carcinoma cell-line HGC-27. As a result, the high plasmid DNA binding affinity, low cytotoxicity, and much enhanced transgene efficacy suggest a new possible clue to design effective synthetic gene delivery vectors with amphiphilic skeleton and less toxic polyamine building blocks.
语种: 英语
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WOS记录号: WOS:000268661400034
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/22940
Appears in Collections:高分子材料研究室_期刊论文

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Recommended Citation:
Li Y,Zhu YD,Xia KJ,et al. Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification[J]. Biomacromolecules,2009,10(8):2284-2293.
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