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Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification
其他题名聚乳酸-树枝状聚赖氨酸两亲基因载体的研究:赖氨酸代数的作用以及端基修饰增强转基因效率
Li Y(李洋); Zhu YD(朱英丹); Xia KJ(夏可嘉); Sheng RL(盛瑞隆); Jia L(贾林); Hou XD(侯小东); Xu YH(徐宇虹); Cao AM(曹阿民)
2009
发表期刊Biomacromolecules
ISSN1525-7797
卷号10期号:8页码:2284-2293
摘要As an effort to prepare new efficient gene delivery vectors, we have recently developed and reported an amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine) D-2-PLLA-D-2 with two-generation PLL dendrons and a PLLA block. In this work, we continued to explore the roles of dendritic PLL generation in DNA binding and intracellular delivery of gene, and a new series of amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine)s D-n-PLLA-D-n (n = 3-5) were synthesized and were structurally characterized. Furthermore, plasmid DNA binding affinity for these cationic amphiphiles was examined by agarose gel electrophoresis and fluorescence titration assay in pure water and PBS buffer solution containing 150 mM NaCl (pH = 7.4), respectively. By dynamic light scattering (DLS) and transmission electronic microscopy (TEM), the interaction and complexation in between were investigated, concerning the DNA/vector polyplex particle morphologies and zeta potentials. Utilizing a human hepatocellular carcinoma cell-line SMMC-7721, cell toxicity, and gene transfection in vitro were explored. To further improve transgene efficiency for these synthetic cationic amphiphiles as gene delivery vectors, new structural DEn-PLLA-DEn (n = 2-3) were prepared through an amino termini modification of the D-n-PLLA-D-n (n = 2-3) with less toxic 4,7,10,13-tetraazatridecanoic acids, and gene transfection with these DEn-PLLA-DEn (n = 2-3) was examined with an alternative human gastric carcinoma cell-line HGC-27. As a result, the high plasmid DNA binding affinity, low cytotoxicity, and much enhanced transgene efficacy suggest a new possible clue to design effective synthetic gene delivery vectors with amphiphilic skeleton and less toxic polyamine building blocks.
学科领域高分子化学
部门归属上海有机所; 上海交通大学
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000268661400034
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/22940
专题高分子材料研究室
通讯作者Xu YH(徐宇虹); Cao AM(曹阿民)
推荐引用方式
GB/T 7714
Li Y,Zhu YD,Xia KJ,et al. Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification[J]. Biomacromolecules,2009,10(8):2284-2293.
APA 李洋.,朱英丹.,夏可嘉.,盛瑞隆.,贾林.,...&曹阿民.(2009).Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification.Biomacromolecules,10(8),2284-2293.
MLA 李洋,et al."Dendritic Poly(L-lysine)-b-Poly(L-lactide)-b-Dendritic Poly(L-lysine) Amphiphilic Gene Delivery Vectors: Roles of PLL Dendritic Generation and Enhanced Transgene Efficacies via Termini Modification".Biomacromolecules 10.8(2009):2284-2293.
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