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学科主题: 生命有机化学
题名: 白术糖复合物AMP-B的理化性质及降血糖活性的研究
其他题名: Studies on physico-chemical properties and hypolycemic activity of complex polysaccharde AMP-B from atractylodes macrocephala koidz
作者: 单俊杰 ; 田庚元
通讯作者: 田庚元
刊名: 药学学报
发表日期: 2003-01-01
卷: 38, 期:6, 页:438-441
部门归属: 中国科学院上海有机化学研究所生命有机国家重点实验室
摘要: 目的研究白术糖复合物AMP—B的理化性质及其对正常和糖尿病大鼠降血糖的作用。方法用水浸、醇沉、膜分离和冷冻干燥得到粗多糖,再经柱色谱得白术糖复合物AMP—B.建立大鼠四氧嘧啶糖尿病模型,分3个剂量组给药,测定血糖值、饮水量和耗食量,及胸腺指数、胰腺指数。结果 AMP—B是一种糖复合物,各元素质量分数为:C32.84%, H5.68%, N 1.79%。碳水化合物50.3%,糖醛酸40.4%,蛋白质11.5%。气相色谱分析表明 AMP—B由葡萄糖、半乳糖、甘露糖、阿拉伯糖和鼠李糖组成,摩尔比3.0:2.5:1.3:3.5:1.0。 AMP—B50,100和200 mg.kg~-1 ig能显著降低四氧嘧啶糖尿病大鼠血糖水平,与模型组比较p<0.01,对正常大鼠血糖无影响。 AMP—B能减少糖尿病大鼠的饮水量和耗食量,对四氧嘧啶引起的大鼠胰岛损伤有一定的恢复作用,抵抗四氧嘧啶引起的大鼠胸腺、胰腺萎缩。结论 AMP—B对四氧嘧啶引起的糖尿病大鼠有明显降血糖作用。
英文摘要: Aim To isolate a complex polysaccharide ( AMP-B ) from ,Atractylocles macrocephhala Koidz and study its phtsico-chemical properties and hypoglycemic activity . Methods The root of Atractylodes macrocephala K . Was extracted with water and precipitated with ethanol , dialyzed against water and freeze-dried to get the crude polysaccharides ( AMP) . A complex polysaccharide (AMP-B) was isolated and purified on DEAE_cellulose column . The model of diabeles rats was established wilh alloxan injection through the tail vein . Male rats were divided into 5 groups: the normal group, the control group, and three AMP-B-fed groups. Measuring the blood glucose, water and food consumplion, thymus and pancreas index, and studying cut ections of pancreas tissues . Results AMP-B is a Compplex-polysaccharide , elementaL anaLysis of AMP-B shown C 32.84% , H 5.68% , and N l .79% . The neutral polysaccharide content of AMP-B was 50.3% , uronic acid was 40.4% , and protein was 11 .5% . Monosaccharide composition of AMP-B was determined by GC, AMP-B composed of Glc, Gal, Man, Ara and Rha in a molar ratio of 3.O:2.5 : l .3:3.5: 1 .0. AMP-B was found to reduce blood glucose level in alloxan-diabetic rats markedly at doses of 50 , 1OO and 200 mg' kg- 1 by ig, but no effect in nonnal rat . AMP-B was found to decrease the consumption of water and food , recover pancreas damage of diabetic rats obviously , inhibited the atrophy of thymus and pancreas of the diabetic rats induced by alloxan . Conelusion AMP-B showed signiffcant hypoglycemic effeot on the experimental hyperglycemias rats indnced by alloxan .
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/22742
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
单俊杰,田庚元. 白术糖复合物AMP-B的理化性质及降血糖活性的研究[J]. 药学学报,2003,38(6):438-441.
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