SIOC OpenIR  > 生命有机化学国家重点实验室
钝顶螺旋藻多糖SPPC—1的分离纯化,性质及活性的研究
Alternative TitleIsolation, purification, physico-chemical properties and biological activities of a polysaccharide SPPC-1 from Spirulina platensis
王仲孚; 彭志英; 黄琳娟; 田庚元
2001-01-01
Source Publication中国海洋药物
ISSN1002-3461
Issue3Pages:17-27
Abstract采用丙酮分部沉淀三氯乙酸(TCA)去蛋白再经SephadexG—75和CM—SephadexC—50柱层析从钝顶螺旋藻中分离纶化得到一种多糖化合物SPPC-1。经高压液相色谱和毛细管电泳鉴定为均一性组分。疑胶过滤法测得其相对分子质量为7.4×10^4。糖组成分析表明其主要由鼠李糖组成,还含有少量的木糖、甘露糖和葡萄糖,摩尔比为 Rha:Xyl:Man:Glc=6.24:O.37;O.13,0.71。元素分折表明,SPPC—1含碳42.59%.含氢8.07%,红外光谱分析表明,SPPC—1含α-型糖苷连接键。ESR研究表明SPPA—l具有清除O^-2自由基活性。
Other AbstractA polysaccharide SPPC-1 was isolated from spirulina platensis by acetone fractional precipation, free protein was removed by trichlorocetic acid (TCA). Purification of the polysaccharide by Sephadex G-75 colume and CM-Sephadex C-50 colume. The homogeneity of this polysaccharide was proved HPLC and CE. Molecular weight was 1.62×10^6. GC showed that the glycoconjugate was composed of Rha, Xyl, Man and Glc. Molar ration is Rha: Xyl: Man:Glc=6.24:0.37:0.13; 0.71. IR, ~1HNMR spectrum indicated the presence of α-linkage glycoside. ESR experiment showed that SPPC-1 could eliminate O^-2radicals.
Subject Area生命有机化学
Department华南理工大学生物科学与工程研究中心; 中国科学院上海有机化学研究所生命有机国家开放实验室
Language中文
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/22714
Collection生命有机化学国家重点实验室
Corresponding Author田庚元
Recommended Citation
GB/T 7714
王仲孚,彭志英,黄琳娟,等. 钝顶螺旋藻多糖SPPC—1的分离纯化,性质及活性的研究[J]. 中国海洋药物,2001(3):17-27.
APA 王仲孚,彭志英,黄琳娟,&田庚元.(2001).钝顶螺旋藻多糖SPPC—1的分离纯化,性质及活性的研究.中国海洋药物(3),17-27.
MLA 王仲孚,et al."钝顶螺旋藻多糖SPPC—1的分离纯化,性质及活性的研究".中国海洋药物 .3(2001):17-27.
Files in This Item:
File Name/Size DocType Version Access License
2001010.pdf(299KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[王仲孚]'s Articles
[彭志英]'s Articles
[黄琳娟]'s Articles
Baidu academic
Similar articles in Baidu academic
[王仲孚]'s Articles
[彭志英]'s Articles
[黄琳娟]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[王仲孚]'s Articles
[彭志英]'s Articles
[黄琳娟]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.