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学科主题: 生命有机化学
题名: Primary structure of β-momorcharin, a ribosome-inactivating protein from the seeds of momordica Charantia Linn (Cucurbitaceae)
其他题名: 从苦瓜子中得到一个核糖体失活蛋白β-苦瓜子蛋白的一级结构
作者: Ye GJ(叶国杰) ; Lu BY(卢保元) ; Jin SW(金善炜) ; Qian RQ(钱瑞卿) ; Wang Y(汪猷)
通讯作者: 叶国杰
刊名: Chin. J. Chem.
发表日期: 1999-01-01
卷: 17, 期:6, 页:658-673
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所
英文摘要: The complete amino acid sequence of β-momorcharin, a ribosome-inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae) has been determined. This has been done by the sequence analysis of peptides obtained by enzymatic digestion with trypsin, chymotrypsin and S. Aureus V8 protease, as well as by chemical cleavage with BNPS-skatole. The protein consists of 249 amino acid residues containing one asparagine-linked sugar group attached to the site of Asn 51 and has a calculated relative molecular mass of 28,452 Da without addition of the carbohydrate. Comparison of this sequence with those of trichosanthin and other ribosome-inactivating proteins from different species of plants shows a significant homology with each other. Regarding the similarity of their biological properties, and active domain of these proteins has been predicted here.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/21990
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Ye GJ,Lu BY,Jin SW,et al. Primary structure of β-momorcharin, a ribosome-inactivating protein from the seeds of momordica Charantia Linn (Cucurbitaceae)[J]. Chin. J. Chem.,1999,17(6):658-673.
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