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学科主题: 生命有机化学
题名: Generation of trans-arachidonic acid under nitrative stress is associated with upregulation of thromponsdin-1 in diabetic rats
其他题名: 硝化应激下反式花生四烯酸的生成与糖尿病大鼠中凝血栓蛋白1的上调相关
作者: Xu L(许琳) ; Liu YM(刘玉敏) ; Yao ZJ(姚祝军) ; Xun GL(寻国良) ; YU WENJUAN ; LIU KUN ; SUN XIAODONG ; QIU YUNPING ; WU HAIXIANG ; Xu X(许迅)
通讯作者: 许迅
刊名: Chin. Med. J.
发表日期: 2011
卷: 124, 期:12, 页:1885-1889
收录类别: SCI
部门归属: 上海交通大学附属第一人民医院; 上海市科委重点实验室上海市眼底病实验室; 上海交通大学; 上海交通大学药学院; 中科院上海有机化学研究所
英文摘要: Background Trans-arachidonic acids (TAAs), newly discovered markers of nitrative stress and the major products of nitrogen dioxide (NO(2)(-))-mediated isomerization of arachidonic acid (AA), represent a new mechanism of NO(2)(-)-induced toxicity. It has been reported that TAAs were generated in oxygen-induced microvascular degeneration model and TAAs were also generated in a diabetic retinopathy (DR) model. In this study, we examined high glucose-induced nitrative stress damage and TAAs levels and explored the possible mechanisms for DR caused by reactive nitrogen species. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ) at 60 mg/kg. Bovine retinal capillary endothelial cells (BRECs) were selectively cultured and incubated with normal or high glucose. The serum TAAs and AA in diabetic rats were measured by the gas chromatography and mass spectrometry (GC/MS) method. The ratio of peak area of TAAs to AA with selected ion of 79 was estimated by a group t-test. Thrombospondin-1 (TSP-1) in the rat retinas and BRECs extracts were examined by Western blotting. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) protein was examined by Western blotting in BRECs incubated with high glucose. Results The TAAs to AA ratio (TAAs/AA) was significantly increased in the serum at 8, 12 and 16 weeks after STZ injection (P <0.05), with no noticeable change found at 2 or 4 weeks (P >0.05). Expression of TSP-1 in the retina of diabetic rats was progressively elevated according to the duration of diabetes. TSP-1 expression was increased in BRECs incubated with high glucose at 48 hours. Moreover, high glucose also increased ERK1/2 expression, which peaked at 30 minutes and then decreased in the following 48 hours. Conclusion An elevation of TAAs/AA is associated with high glucose-induced nitrative stress, which probably involves upregulation of TSP-1 through activating ERK1/2. Chin Med J 2011;124(12):1885-1889
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000292350100023
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/21155
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Xu L,Liu YM,Yao ZJ,et al. Generation of trans-arachidonic acid under nitrative stress is associated with upregulation of thromponsdin-1 in diabetic rats[J]. Chin. Med. J.,2011,124(12):1885-1889.
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