中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 生命有机化学国家重点实验室  > 期刊论文
学科主题: 生命有机化学
题名: In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer
其他题名: FRET实验观察NodD和诱导信号分子柚皮素的体外相互作用
作者: Li FQ(李风庆) ; Hou BH(侯碧和) ; Chen L(陈磊) ; Yao ZJ(姚祝军) ; Hong GF(洪国藩)
通讯作者: 洪国藩
刊名: Acta Biochim. Biophys. Sin.
发表日期: 2008
卷: 40, 期:9, 页:783-789
收录类别: SCI
部门归属: 上海生科院生化细胞所; 上海有机所
英文摘要: At initial stages in the Rhizobium legume symbiosis, most Modulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing interaction. In this paper, we used fluorescence resonance energy transfer technique to see whether such an interaction exists between NodD and its activator, naringenin, in vitro. The tetracysteine motif (Cys-Cys-Pro-Gly-Cys-Cys) was genetically inserted into NodD to label NodD with 4',5'-bis(1,3,2-dithioarsolan-2-yl) fluorescein (FlAsH). Naringenin was labeled with fluorescein by chemical linking. In the fluorescence resonance energy transfer experiments in vitro, the fluorescence intensity of one acceptor, NodD(90R6)-FlAsH, increased by 13%. This suggests that NodD may directly interact with inducer naringenin in vitro and that the reaction centre is likely near hinge region 1 of NodD.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000259676100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/21111
Appears in Collections:生命有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2008092.pdf(271KB)----开放获取--View Download

Recommended Citation:
Li FQ,Hou BH,Chen L,et al. In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer[J]. Acta Biochim. Biophys. Sin.,2008,40(9):783-789.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[李风庆]'s Articles
[侯碧和]'s Articles
[陈磊]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[李风庆]‘s Articles
[侯碧和]‘s Articles
[陈磊]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2008092.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace