中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 分析化学研究室  > 期刊论文
学科主题: 分析化学
题名: Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization
其他题名: N-烷基吡啶同位素季铵化反应结合基质辅助激光解析傅里叶变换质谱分析应用的改进和扩展
作者: Wang H(汪航) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Leng JP(冷嘉鹏) ; Cai TT(蔡婷婷) ; Guo YL(郭寅龙)
通讯作者: 郭寅龙
刊名: Anal. Chim. Acta
发表日期: 2011
卷: 707, 期:1, 页:100-106
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: Based on our previous report on N-alkylpyridinium isotope quaternization (NAPIQ) for the analysis of cholesterol and fatty alcohols, we have further developed the NAPIQ method for wider applications in screening various small compounds. The reaction scope and improvement were investigated via the screening of compounds with different reaction sites. The experimental results showed that the NAPIQ strategy was suitable not only for steroids with alcoholic or alpha,beta-unsaturated ketone moieties, but could also be applied to label carbohydrates by changing the reaction solvent. The byproducts from target compounds with multiple groups could be reduced by improving the reaction conditions. The derivatization method significantly improved the detection sensitivity for these compounds in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric (MALDI-FTMS) analysis. The detection sensitivity and selectivity of the NAPIQ method were higher than conventionally used Girard reagents. Finally, endogenous steroids in urine samples were screened using the NAPIQ method. NAPIQ was proven to be an efficient alternative method for analyzing steroids and carbohydrates, without the need for isotopically coded internal standards. (C) 2011 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000297141800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/20060
Appears in Collections:分析化学研究室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2011518.pdf(639KB)----开放获取--View Download

Recommended Citation:
Wang H,Wang HY,Zhang L,et al. Improvement and extension of the application scope for matrix-assisted laser desorption/ionization mass spectrometric analysis-oriented N-alkylpyridinium isotope quaternization[J]. Anal. Chim. Acta,2011,707(1):100-106.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[汪航]'s Articles
[王昊阳]'s Articles
[张立]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[汪航]‘s Articles
[王昊阳]‘s Articles
[张立]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2011518.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace