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学科主题: 分析化学
题名: Assisted inhibition effect of acetylcholinesterase with n-octylphosphonic acid and application in high sensitive detection of organophosphorous pesticides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry
其他题名: 正辛基磷酸辅助抑制乙酰胆碱酯酶及其结合基质辅助激光解析离子化质谱高灵敏度地检测有机磷农药的应用
作者: Cai TT(蔡婷婷) ; Zhang L(张立) ; Wang HY(王昊阳) ; Zhang J(张菁) ; Guo YL(郭寅龙)
通讯作者: 张立 ; 郭寅龙
刊名: Anal. Chim. Acta
发表日期: 2011
卷: 706, 期:2, 页:291-296
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: A simple and practical approach to improve the sensitivity of acetylcholinesterase (AChE)-inhibited method has been developed for monitoring organophosphorous (OP) pesticide residues. In this work, matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) was used to detect AChE activity. Due to its good salt-tolerance and low sample consumption, MALDI-FTMS facilitates rapid and high-throughput screening of OP pesticides. Here we describe a new method to obtain low detection limits via employing external reagents. Among candidate compounds, n-octylphosphonic acid (n-Octyl-PA) displays assistant effect to enhance AChE inhibition by OP pesticides. In presence of n-Octyl-PA, the percentages of AChE inhibition still kept correlation with OP pesticide concentrations. The detection limits were improved significantly even by 10(2)-10(3) folds in comparison with conventional enzyme-inhibited methods. Different detection limits of OP pesticides with different toxicities were as low as 0.005 mu g L(-1) for high toxic pesticides and 0.05 mu g L(-1) for low toxic pesticides. Besides, the reliability of results from this method to analyze cowpea samples had been demonstrated by liquid-chromatography tandem mass spectrometry (LC-MS/MS). The application of this commercial available assistant agent shows great promise to detect OP compounds in complicated biological matrix and broadens the mind for high sensitivity detection of OP pesticide residues in agricultural products. (C) 2011 Elsevier B.V. All rights reserved.
语种: 英语
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WOS记录号: WOS:000297184600012
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/20058
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Cai TT,Zhang L,Wang HY,et al. Assisted inhibition effect of acetylcholinesterase with n-octylphosphonic acid and application in high sensitive detection of organophosphorous pesticides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry[J]. Anal. Chim. Acta,2011,706(2):291-296.
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