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学科主题: 分析化学
题名: N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair
其他题名: N-烷基吡啶同位素季铵化反应结合基质辅助激光解析傅里叶变换质谱分析人毛发中的胆固醇和脂肪醇
作者: Wang H(汪航) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Guo YL(郭寅龙)
通讯作者: 郭寅龙
刊名: Anal. Chim. Acta
发表日期: 2011
卷: 690, 期:1, 页:1-9
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: Isotope-coded reagents have been developed for labeling of amino acids, phenols and fatty acids, but not for alcohols. In this work, a simple reaction based on direct N-alkylpyridinium isotope quaternization (NAPIQ) was developed for mild derivatization of cholesterol and fatty alcohols. Different from the conventional quaternary reagents with cations on themselves, two simple and charge-neutral reagents: pyridine and d(5)-pyridine directly attached N-cationic tag onto the target compounds in the presence of trifluoromethanesulfonic anhydride (Tf(2)O) without tedious sample preparation. The derivatization completed in 5 min and achieved charge labeling of the target compounds, which significantly improved the detection limits of analytes by 10(3)-folds in further analysis by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS). The use of commercially available d(0)/d(5)-pyridine pairs facilitated isotope-coded chemical derivatization and avoided the use of isotope-labeled internal standards; the excess pyridine did not affect the signals of analytes. Utility of the NAPIQ method was examined in the identification of cholesterol and fatty alcohols in small amount of human hair sample (<0.5 mg). The fluctuation of total cholesterol in human body was profiled during time by quantitatively comparing the different segments of a single strand of hair. This study combines the direct pyridinium quaternization with MALDI-FTMS, which offers a perspective and an alternative tool for the identification and quantification of substances in biological matrix by comparing d(0/)d(5) pairs, especially when isotope-labeled internal standards are unavailable. (C) 2011 Elsevier B.V. All rights reserved.
语种: 英语
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WOS记录号: WOS:000289131500001
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/20040
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Wang H,Wang HY,Zhang L,et al. N-Alkylpyridinium isotope quaternization for matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of cholesterol and fatty alcohols in human hair[J]. Anal. Chim. Acta,2011,690(1):1-9.
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