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学科主题: 分析化学
题名: Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS
其他题名: 应用异硫氰酸基同位素标记试剂结合电喷雾离子阱飞行时间质谱进行高准确性的多肽测序以及蛋白质定量分析
作者: Leng JP(冷嘉鹏) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Wang H(汪航) ; Cai TT(蔡婷婷) ; Yao JT(姚劲挺) ; Guo YL(郭寅龙)
通讯作者: 郭寅龙
刊名: J. Am. Soc. Mass Spectrom.
发表日期: 2011
卷: 22, 期:7, 页:1204-1213
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: A multifunctional isothiocyanate-based isotope labeling reagent, [d (0)]-/[d (6)]-4,6-dimethoxy pyrimidine-2-isothiocyanate (DMPITC), has been developed for accurate N-terminus identification in peptide sequencing and comparative protein analysis by ESI Ion-trap TOF mass spectrometry. In contrast with the conventional labeling reagent phenyl isothiocyanate (PITC), DMPITC showed more desirable properties such as rapid labeling, sensitivity enhancement, and facilitating peptide sequencing. More significantly, DMPITC-based labeling strategy possessed the capacity of higher reliable N-terminus identification owning to the high-yield b(1) ion combined with the isotope validation of 6 Da. Meanwhile, it also showed potential in differentiating isomeric residues of leucine and isoleucine at N-terminus on the basis of the relative abundance ratios between the fragment ions of their respective b(1) ions. The strategy not only allows accurate interpretation for peptide but also ensures rapid and sensitive comparative analysis for protein by direct MS analysis. Using trypsin-digested bovine serum albumin (BSA), both peptide N-terminus identification and quantitative analysis were accomplished with high accuracy, efficiency, and reproducibility. The application of DMPITC-based labeling strategy is expected to serve as a promising tool for proteome research.
语种: 英语
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WOS记录号: WOS:000291257400011
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/20036
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Leng JP,Wang HY,Zhang L,et al. Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS[J]. J. Am. Soc. Mass Spectrom.,2011,22(7):1204-1213.
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