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Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry
其他题名B型肝炎患者舌苔中角蛋白的质谱法研究
Fang F(方芳); Liu P(刘平); Wang HY(王昊阳); Zhang L(张立); Zhang J(张菁); Gao YP(高玉平); Ceng LM(曾丽敏); Guo YL(郭寅龙)
2009
发表期刊Rapid Commun. Mass Spectrom.
ISSN0951-4198
卷号23期号:11页码:1703-1709
摘要Pooled tongue coating samples from 64 hepatitis B patients and 24 healthy adults were studied and a major band of differential proteins was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The differential proteins in this band were identified and proved to be keratins by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and Western blot analysis. Furthermore, relative quantification of the identified keratins was performed via using stable isotopic labeling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), showing the higher expression level of these keratins in tongue coating samples of hepatitis B patients than healthy adults. These results provided additional information to understand the medical diagnosis depending on the tongue coating. Copyright (C) 2009 John Wiley & Sons, Ltd.
学科领域分析化学
部门归属上海有机所; 上海中医药大学
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收录类别SCI
语种英语
WOS记录号WOS:000266724000020
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/19982
专题分析化学研究室
通讯作者Liu P(刘平); Guo YL(郭寅龙)
推荐引用方式
GB/T 7714
Fang F,Liu P,Wang HY,et al. Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry[J]. Rapid Commun. Mass Spectrom.,2009,23(11):1703-1709.
APA 方芳.,刘平.,王昊阳.,张立.,张菁.,...&郭寅龙.(2009).Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry.Rapid Commun. Mass Spectrom.,23(11),1703-1709.
MLA 方芳,et al."Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry".Rapid Commun. Mass Spectrom. 23.11(2009):1703-1709.
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