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学科主题: 分析化学
题名: Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry
其他题名: B型肝炎患者舌苔中角蛋白的质谱法研究
作者: Fang F(方芳) ; Liu P(刘平) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Gao YP(高玉平) ; Ceng LM(曾丽敏) ; Guo YL(郭寅龙)
通讯作者: 刘平 ; 郭寅龙
刊名: Rapid Commun. Mass Spectrom.
发表日期: 2009
卷: 23, 期:11, 页:1703-1709
收录类别: SCI
部门归属: 上海有机所; 上海中医药大学
英文摘要: Pooled tongue coating samples from 64 hepatitis B patients and 24 healthy adults were studied and a major band of differential proteins was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The differential proteins in this band were identified and proved to be keratins by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and Western blot analysis. Furthermore, relative quantification of the identified keratins was performed via using stable isotopic labeling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), showing the higher expression level of these keratins in tongue coating samples of hepatitis B patients than healthy adults. These results provided additional information to understand the medical diagnosis depending on the tongue coating. Copyright (C) 2009 John Wiley & Sons, Ltd.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000266724000020
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19982
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Fang F,Liu P,Wang HY,et al. Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry[J]. Rapid Commun. Mass Spectrom.,2009,23(11):1703-1709.
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