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学科主题: 分析化学
题名: Highly efficient and of selective enrichment phosphopeptides using porous anodic alumina membrane for MALDI-TOF MS analysis
其他题名: 应用多孔铝膜高效高选择性富集磷酸化肽用于MALDI-TOF MS分析
作者: Wang YB(王粤博) ; Chen W(陈伟) ; Wu JS(吴建爽) ; Guo YL(郭寅龙) ; Xia XH(夏兴华)
通讯作者: 郭寅龙 ; 夏兴华
刊名: J. Am. Soc. Mass Spectrom.
发表日期: 2007
卷: 18, 期:8, 页:1387-1395
收录类别: SCI
部门归属: 南京大学; 上海有机所上海质谱中心
英文摘要: Because of its good biocompatibility, high surface-to-volume ratio, and distinct surface electrical properties, porous anodic alumina (PAA) membrane has been used to selectively enrich phosphopeptides from a mixture of synthetic peptides and tryptic digest product of beta-casein by a direct MALDI-TOF MS analysis. As we reported previously, PAA membrane has strong incorporation ability to the phosphate anion. Herein, we describe the application of PAA membrane as a selective sampling absorbent for phosphopeptides. The PAA membrane could enrich phosphopeptides with high efficiency and selectivity; for example, the tryptic digest product of beta-casein at a concentration as low as 4 X 10(-9) M can be satisfactorily detected. Compared to that from the nonenriching peptide mixture, the MS signal of the phosphorylated peptides enriched by the PAA membrane is remarkably improved. In addition, acidic peptides have insignificant influence on the enriching process. Results show that the adsorption of phosphate anions on the PAA membrane plays a determining role in achieving highly selective enriching capacity toward phosphopeptides. The feasibility of PAA membranes as specific absorbents for phosphopeptides is also demonstrated.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000248603700004
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19950
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Wang YB,Chen W,Wu JS,et al. Highly efficient and of selective enrichment phosphopeptides using porous anodic alumina membrane for MALDI-TOF MS analysis[J]. J. Am. Soc. Mass Spectrom.,2007,18(8):1387-1395.
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