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学科主题: 分析化学
题名: Carbon nanotubes (2,5-dihydroxybenzoyl hydrazine) derivative as pH adjustable enriching reagent and matrix for MALDI analysis of trace peptides
其他题名: 碳纳米管2,5-二羟基苯甲酰肼衍生物作为pH可调节的富集试剂及基质对微量肽分析的研究
作者: Ren SF(任士芳) ; Guo YL(郭寅龙)
通讯作者: 郭寅龙
刊名: J. Am. Soc. Mass Spectrom.
发表日期: 2006
卷: 17, 期:7, 页:1023-1027
收录类别: SCI
部门归属: 上海有机所上海质谱中心
英文摘要: A functionalized carbon nanotube (CNT), CNT 2,5-clihydroxy-benzoyl hvdrazine derivative, was synthesized and used as both pH adjustable enriching reagent and matrix in matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis of trace peptides. The derivative reagent, 2,5-dihydroxybenzovl hydrazine, introduced phenolic hydroxyl and phenyl groups to the surface of the CNT. The former group can provide adjustable surface charge and a source of protons for chemical ionization, and the latter helps to keep strong ultraviolet absorption for enhancing pulsed laser desorption and ionization. It was found that the functionalized CNT was less twisted in a basic condition (pH 10.5), which afforded an increased surface area to Volume ratio for adsorption towards trace peptides. However, functionalized CNT becomes deposited in an acidic condition (pH 5) and can be isolated readily from the sample solutions once the nanoparticles have trapped the target analytes, thus providing a novel and convenient alternative method for quick isolation. Compared with the previously reported method on enriching analytes using the pristine CNT, it is observed that the detection limit for analytes can be greatly improved due to enhancing adsorption capacity of the functionalized CNT. Moreover, peptide Mixture at concentration as low as 0.01 pg/mu L still can be detected after enrichment mediated by the functionalized CNT, while it is difficult to be detected without enrichment at concentration 0.1 pg/mu L using alpha-cyano-4-hydroxycinnamic acid (CHCA) as matrix. Therefore, high efficiency of adsorption and enrichment towards trace peptides can be achieved by adjusting pH value of the functionalized CNT dispersion.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000238869900018
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19922
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Ren SF,Guo YL. Carbon nanotubes (2,5-dihydroxybenzoyl hydrazine) derivative as pH adjustable enriching reagent and matrix for MALDI analysis of trace peptides[J]. J. Am. Soc. Mass Spectrom.,2006,17(7):1023-1027.
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