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学科主题: 生命有机化学
题名: Six specific lysine residues are crucial in maintaining the structure and function of soluble manganese stabilizing protein spectrum; manganese stabilizing protein; Lys residue
其他题名: 维持水溶液中锰稳定蛋白结构和功能的六个赖氨酸残基
作者: Gao JP(高金鹏) ; Zhang F(张峰) ; Zhang L(张立) ; Guo YL(郭寅龙) ; Ruan CK(阮承康) ; Jiang DA(姜德安) ; Xu CH(徐春和)
通讯作者: 姜德安 ; 徐春和
刊名: Acta Biochim. Biophys. Sin.
发表日期: 2006-01-01T00:00:00Z
卷: 38, 期:9, 页:611-619
收录类别: SCI
部门归属: 中科院上海植物生态研究所; 中国科学院研究生院; 浙江大学; 上海有机所上海质谱中心; 中科院生理生化研究所
英文摘要: When manganese stabilizing protein (MSP) was treated with 0.5 mM N-succinimidyl propionate (NSP), the rebinding ability and oxygen-releasing capabilities of the modified MSP were not altered, in spite of changes of MSP surface Lys residues. Furthermore, far-ultraviolet circular dichroism and intrinsic fluorescence spectra analysis revealed that 0.5 mM NSP-modified MSP retained most of its native secondary and tertiary structure. Mapping of the sites of NSP modification by Staphylococcus V-8 protease digestion of the modified protein, as well as analysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry, indicated that seven Lys residues were modified. The results suggested that these residues are not absolutely essential to the structure and function of MSP. However, when the NSP concentration was increased to 4 mM, the modified MSP was unable to bind photosystem II and completely lost its reactivating capability. Both far-ultraviolet circular dichroism and intrinsic fluorescence spectra analysis revealed a clear conformational change in MSP after 4 mM NSP treatment, suggesting that some Lys residues are involved in maintaining the structure and function of MSP. Analysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry indicated that another six Lys residues, namely Lys20, Lys101, Lys196, Lys207, Lys130 (or Lys137) and Lys66 (or Lys76), were modified by 4 mM NSP. Therefore, these six Lys residues are crucial in maintaining the structure and function of soluble MSP.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19918
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Gao JP,Zhang F,Zhang L,et al. Six specific lysine residues are crucial in maintaining the structure and function of soluble manganese stabilizing protein spectrum; manganese stabilizing protein; Lys residue[J]. Acta Biochim. Biophys. Sin.,2006,38(9):611-619.
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