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Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates
其他题名固定化碳纳米管作为基质用于MALDI-TOF质谱分析: 应用于中性小分子寡糖分析
Ren SF(任士芳); Zhang L(张立); Cheng ZH(程志红); Guo YL(郭寅龙)
2005
发表期刊J. Am. Soc. Mass Spectrom.
ISSN1044-0305
卷号16期号:3页码:333-339
摘要In this work, we reported on the advantages of immobilized carbon nanotubes as a novel MALDI-matrix. Recently, carbon nanotubes have been reported to be an effective MALDI matrix for small molecules (Anal. Chem 2003, 75, 6191), as it can eliminate the interfering matrix peaks as well as form a web morphology to fully disperse the analyte and allow strong ultraviolet absorption for enhanced pulsed laser desorption and ionization. In our study, to overcome the problem that the carbon nanotube matrix may fly off from the target, a type of polyurethane adhesive, NIPPOLAN-DC-205, is introduced to immobilize carbon nanotubes on the target, which enables widespread application of carbon nanotubes as matrix for MALDI-MS analysis. At the same time, the properties of the carbon nanotubes as an efficient matrix remained after immobilization. The presence of NIPPOLAN-DC-205 increases the time for analysis at a particular desorption spot by minimizing the time-consuming search for "hot spots" and facilitating experiments such as post source decay (PSD) which need longer-lasting signals. Moreover, NIPPOLAN-DC-205 produces no interference peaks and can easily be cleaned with acetone. Fast evaporation technology may be used to enhance signal reproducibility in MALDI analysis using carbon nanotubes as matrix. Consequently, the applicability of the carbon nanotube as matrix for matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS) analysis of low molecular mass analytes is highly improved. The feasibility of the method employing polyurethane is demonstrated by comparison of the results produced from the carbon nanotube matrix with and without immobilization. In addition, neutral small carbohydrates, which are difficult to be ionized normally, can be cationized with high efficiency by MALDI-TOF-MS using the immobilized carbon nanotube matrix. The method was further applied to analyze peptides and detect urine glucose successfully. (J Am Soc Mass Spectrom 2005, 16, 333-339) (C) 2005 American Society for Mass Spectrometry.
学科领域分析化学
部门归属有机化学研究所
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收录类别SCI
语种英语
WOS记录号WOS:000227402000006
引用统计
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/19902
专题分析化学研究室
通讯作者Guo YL(郭寅龙)
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Ren SF,Zhang L,Cheng ZH,et al. Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates[J]. J. Am. Soc. Mass Spectrom.,2005,16(3):333-339.
APA 任士芳,张立,程志红,&郭寅龙.(2005).Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates.J. Am. Soc. Mass Spectrom.,16(3),333-339.
MLA 任士芳,et al."Immobilized carbon nanotubes as matrix for MALDI-TOF-MS analysis: Applications to neutral small carbohydrates".J. Am. Soc. Mass Spectrom. 16.3(2005):333-339.
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