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学科主题: 分析化学
题名: Acetonitrile-induced unfolding of the photosystem II manganese-stabilizing protein studied by electrospray mass spectrometry
其他题名: 乙腈诱导光系统II锰稳定蛋白解折叠的电喷雾质谱研究
作者: Zhang L(张立) ; Zhang F(张峰) ; Zhang SY(张素艳) ; Guo YL(郭寅龙) ; Xu CH(徐春和)
通讯作者: 郭寅龙 ; 徐春和
刊名: Rapid Commun. Mass Spectrom.
发表日期: 2005
卷: 19, 期:15, 页:2151-2156
收录类别: SCI
部门归属: 上海有机化学研究所; 中国科学院上海植物生理研究所
英文摘要: In this paper an acetonitrile-induced unfolding of the manganese-stabilizing protein (MSP) of photosystem II was discovered. More distinct unfolding states of MSP were identified than previously by using mainly electrospray ionization mass spectrometry (ESI-MS), together with fluorescence spectra and far-UV circular dichroism (CD) at pH 2.0, 6.2 or 11.6, and with acetonitrile concentrations from 0 to 50%. At pH 6.2 with acetonitrile concentration changing from 0 to 10%, relatively broad charge-state distributions and poor intensity were observed in ESI-MS, indicating the presence of coexisting conformers. It was concluded that the structure of the MSP protein is unlikely to be a tightly folded form. When the concentration of acetonitrile was 20-40%, simulating the state in the biological membrane, changes in the state of unfolding of MSP were observed to a certain extent using ESI-MS, fluorescence and CD spectroscopy. The charge-state distribution in ESI-MS was found to move toward high states (from 13(+) to 27(+) to 15(+) to 31(+)) with increasing acetonitrile concentration. At pH 2.0, the MSP structure is rearranged into an unfolded state, and at pH 11.6 the MSP structure is induced to assume another unordered state by deprotonation of appropriate residues. An interesting observation was that a second peak envelope emerged with 20-50% acetonitrile in the medium at pH 11.6. Copyright (c) 2005 John Wiley & Sons, Ltd.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000230825100007
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19890
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Zhang L,Zhang F,Zhang SY,et al. Acetonitrile-induced unfolding of the photosystem II manganese-stabilizing protein studied by electrospray mass spectrometry[J]. Rapid Commun. Mass Spectrom.,2005,19(15):2151-2156.
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