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学科主题: 分析化学
题名: Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins
其他题名: 多孔氧化铝膜作为样品载体用于基质辅助激光解吸附飞行时间质谱分析含盐蛋白质
作者: Wang YB(王粤博) ; Guo YL(郭寅龙) ; Xia XH(夏兴华)
通讯作者: 王粤博
刊名: J. Am. Soc. Mass Spectrom.
发表日期: 2005
卷: 16, 期:9, 页:1488-1492
收录类别: SCI
部门归属: 上海有机化学研究所; 南京大学
英文摘要: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis of proteins in salt-containing solution was performed for the first time using porous anodic alumina (PAA) membrane as sample support. The resulting spectral quality of proteins under standard sample preparation conditions was superior to that of normal metal sample stages. Analysis of phosphate-doped protein solutions indicated that porous anodic alumina membranes as a target yielded better results than a metallic target for salt-containing solutions. Because of the biocompatibility of the PAA, proteins can be adsorbed on the PAA and thus a washing process can be introduced to remove the salts from the PAA target before MS analysis. This desalting step significantly enhanced spectral quality, and better signal-to-noise ratios were obtained. The present technique is promising for proteomics research.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000231646500010
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19884
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Wang YB,Guo YL,Xia XH. Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins[J]. J. Am. Soc. Mass Spectrom.,2005,16(9):1488-1492.
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