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Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins
其他题名多孔氧化铝膜作为样品载体用于基质辅助激光解吸附飞行时间质谱分析含盐蛋白质
Wang YB(王粤博); Guo YL(郭寅龙); Xia XH(夏兴华)
2005
发表期刊J. Am. Soc. Mass Spectrom.
ISSN1044-0305
卷号16期号:9页码:1488-1492
摘要Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis of proteins in salt-containing solution was performed for the first time using porous anodic alumina (PAA) membrane as sample support. The resulting spectral quality of proteins under standard sample preparation conditions was superior to that of normal metal sample stages. Analysis of phosphate-doped protein solutions indicated that porous anodic alumina membranes as a target yielded better results than a metallic target for salt-containing solutions. Because of the biocompatibility of the PAA, proteins can be adsorbed on the PAA and thus a washing process can be introduced to remove the salts from the PAA target before MS analysis. This desalting step significantly enhanced spectral quality, and better signal-to-noise ratios were obtained. The present technique is promising for proteomics research.
学科领域分析化学
部门归属上海有机化学研究所; 南京大学
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收录类别SCI
语种英语
WOS记录号WOS:000231646500010
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/19884
专题分析化学研究室
通讯作者Wang YB(王粤博)
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Wang YB,Guo YL,Xia XH. Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins[J]. J. Am. Soc. Mass Spectrom.,2005,16(9):1488-1492.
APA 王粤博,郭寅龙,&夏兴华.(2005).Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins.J. Am. Soc. Mass Spectrom.,16(9),1488-1492.
MLA 王粤博,et al."Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins".J. Am. Soc. Mass Spectrom. 16.9(2005):1488-1492.
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