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学科主题: 分析化学
题名: A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis
其他题名: 一种新型含碘树脂衍生物的设计、合成方法及其在基于质谱的蛋白质组学研究中的应用
作者: Zhang L(张立) ; Guo YL(郭寅龙) ; Liu HQ(刘晗青)
通讯作者: 郭寅龙
刊名: J. Mass Spectrom.
发表日期: 2004
卷: 39, 期:4, 页:447-457
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所分析测试中心
英文摘要: A novel class of chemically modified iodo-containing resins with isotope-labeled tagging for mass spectrometry-based proteome analysis is described. This iodo-containing resin contains a thiol-reactive group that is used to capture the cysteine (Cys)-containing peptides from peptide mixtures, one 'tag' amino acid, and an aminomethyl polystyrene resin with Rink Amide Linker. The 'tag' amino acid is synthesized in both heavy and light isotope-coded forms and therefore permits the direct relative quantification of peptides/proteins through mass spectrometric analysis. In the iodo-containing resin strategy, the Cys-containing peptides of two samples covalently captured by either light or heavy iodo-containing resin were mixed and washed extensively under stringent conditions. Then the Cys-containing peptides were retrieved by acid-catalyzed elution. Finally, the eluted peptides were directly analyzed by micro liquid chromatography/mass spectrometry for identification and relative quantification. The iodo-containing resins were synthesized by a simple but effective method. Their abilities to identify and quantify the Cys-containing part in two samples were proved by the analysis of mixtures of amino acids, peptides and proteins. Copyright (C) 2004 John Wiley Sons, Ltd.
语种: 英语
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WOS记录号: WOS:000220860200013
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19862
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Zhang L,Guo YL,Liu HQ. A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis[J]. J. Mass Spectrom.,2004,39(4):447-457.
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