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学科主题: 分析化学
题名: High accuracy mass measurement of peptides with internal calibration using a dual electrospray ionization sprayer system for protein identification
其他题名: 双喷雾内标法蛋白质鉴定中多肽的准确质量测定
作者: Zhou F(周峰) ; Shui WQ(水文清) ; Lu Y(陆宇) ; Yang PY(杨芃原) ; Guo YL(郭寅龙)
通讯作者: 郭寅龙
刊名: Rapid Commun. Mass Spectrom.
发表日期: 2002
卷: 16, 期:6, 页:505-511
收录类别: SCI
部门归属: 复旦大学; 中国科学院上海有机化学研究所分析化学实验室
英文摘要: A dual-ESI-sprayer system was constructed and applied to achieve high accuracy of petide mass measurement for protein identification by means of peptide mapping. Sample was introduced in one sprayer, and reference in the other, thus making internal calibration possible greatly enhancing the mass accuracy. Several samples were utilized to evaluate the reliability of the dual-ESI-sprayer system. The range of mass errors was 0.16-5.37 ppm. The peptide masses of tryptic digests of myoglobin (horse)were measured by the HPLC/dual-ESI-MS system, with mass deviations ranging from 0.01-7.76 ppm, and about 75% mass deviations below 5 ppm with 40% below 1 ppm. These peptide masses were utilized to perform database serching for protein identification, and compared to results obtained by external calibration. This comparison showed that the internal calibration provides a more reliable method of protein indentification, with a much smaller number of required peptides for matching, and with less CPU time consumed for database searching. Copyright.
语种: 英语
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WOS记录号: WOS:000174213700003
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19830
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Zhou F,Shui WQ,Lu Y,et al. High accuracy mass measurement of peptides with internal calibration using a dual electrospray ionization sprayer system for protein identification[J]. Rapid Commun. Mass Spectrom.,2002,16(6):505-511.
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