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学科主题: 生命有机化学
题名: Enhancement of enzyme activity and enantioselectivity via cultivation in nitrile metabolism by rhodococcus sp.CGMCC 0497
其他题名: 增进红球菌CGMCC 0497对腈基化合物代谢的酶活及立体选择性.
作者: Wu ZL(吴中柳) ; Li ZY(李祖义)
通讯作者: 李祖义
刊名: Biotechnol. Appl. Biochem.
发表日期: 2002-01-01
卷: 35, 页:61-67
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室
英文摘要: Racemic 2-phenylpropionitrile was resolved enantioselectively by nitrile-converting enzymes in cells of Rhodococcus sp.CGMCC 0497 to S-(+)-2-phenylpropionic acid and R-(-)-2-phenylpropionamide. By optimization of the culture conditions, great enhancement of enzyme activity and enantioselectivity was achieved. Furthermore, the relationship between cell-growth periodicity and enzyme accumulation was studied; the addition of inducer was delayedby I dayand thereaction was further improved. This unusual strategy has almost never been reported with other nitrile-converting strains. The resulting culture broth, containing methacrylamide as the inducer, beef extract as the nitrogen source and glucose as the carbon souce, with methacrylamide added 24 h later. Seemed to be most suitable S-(+)-2-2Phenylpropionic acid and R(-)-2-phenylpropionamide were produced with yields of 48%(enantiomeric excess, 96%) and 42%(enantiomeric excess, 97%) respectively with no nitrile left after 3 h, or with yields. Of 52% and 39%(enantiomeric excess,93% and 99%) respectively after 6h.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19713
Appears in Collections:金属有机化学国家重点实验室_期刊论文

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Recommended Citation:
Wu ZL,Li ZY. Enhancement of enzyme activity and enantioselectivity via cultivation in nitrile metabolism by rhodococcus sp.CGMCC 0497[J]. Biotechnol. Appl. Biochem.,2002,35:61-67.
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