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学科主题: 生命有机化学
题名: Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2
其他题名: Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2
作者: Jianbei Zhu ; Lei Xiong ; Yu B(俞飚) ; Jiarui Wu
通讯作者: Jiarui Wu
刊名: Mol. Pharmacol.
发表日期: 2005-01-01
卷: 68, 期:6, 页:1831-1838
收录类别: SCI
部门归属: 中科院上海生化细胞所; 中科院上海有机所
英文摘要: OSW-1 is a new member of cholestane saponin family, which is cytotoxic against several types of malignant cells. We reported herein that OSW-1 induced apoptosis of mammalian cells in a concentration- and time-dependent manner. The drug-induced apoptosis was mediated through the mitochondrial pathway, involving the cleavage of Bcl-2. This drug-induced Bcl-2 cleavage in CHO cells could be suppressed either by dominant negative caspase-8 or by a caspase-8 inhibitor, suggesting that the Bcl-2 cleavage is dependent on caspase-8. In contrast, the Bcl-2 cleavage was independent of caspase-3 activity. The inhibition of caspase-8 activity also resulted in the reduction of apoptotic cells, indicating that Bcl-2 cleavage induced by caspase-8 promotes the progression of apoptosis. The involvement of the caspase-8 activity in the processes of the OSW-1 induced apoptosis was further examined by using caspase-8 deficient Jurkat T cells. It was found that the caspase-8 deficient cells were resistant to OSW-1 induced Bcl-2 cleavage or apoptosis. Furthermore, the small subunit of caspase-8 was found to interact with Bcl-2 as determined by yeast two-hybrid and co-immunoprecipitation assays. Overexpression of caspase-8 small subunit reduced the cleavage of Bcl-2 and inhibited the apoptosis induced by OSW-1. Taken together, these results demonstrate that OSW-1 is capable of inducing apoptosis in mammalian cells, in which the caspase-8 dependent cleavage of Bcl-2 plays an important role.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19023
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Jianbei Zhu,Lei Xiong,Yu B,et al. Apoptosis induced by a new member of saponin family is mediated through caspase-8 dependent chevage of Bcl-2[J]. Mol. Pharmacol.,2005,68(6):1831-1838.
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