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学科主题: 生命有机化学
题名: Kinetic analysis of an efficient DNA-dependent TNA polymerase
其他题名: Kinetic analysis of an efficient DNA-dependent TNA polymerase
作者: Allen Horhota ; Keyong Zou ; Justin K. Ichida ; Yu B(俞飚)
通讯作者: 俞飚
刊名: J. Am. Chem. Soc.
发表日期: 2005
卷: 127, 期:20, 页:7427-7434
收录类别: SCI
部门归属: Boston College ; Howard Hughes Medical Institute and Massachusetts General Hospital; 中科院上海有机所
英文摘要: alpha-L-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-D-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs). Therminator DNA polymerase, a variant of the 9 degrees N DNA polymerase, is an efficient DNA-directed threosyl nucleic acid (TNA) polymerase. Here we report a detailed kinetic comparison of Therminator-catalyzed TNA and DNA syntheses. We examined the rate of sing le-nucleotide incorporation for all four tNTPs and dNTPs from a DNA primer-template complex and carried out parallel experiments with a chimeric DNA-TNA primer-DNA template containing five TNA residues at the primer X-terminus. Remarkably, no drop in the rate of TNA incorporation was observed in comparing the DNA-TNA primer to the all-DNA primer, suggesting that few primer-enzyme contacts are lost with a TNA primer. Moreover, comparison of the catalytic efficiency of TNA synthesis relative to DNA synthesis at the downstream positions reveals a difference of no greater than 5-fold in favor of the natural DNA substrate. This disparity becomes negligible when the TNA synthesis reaction mixture is supplemented with 1.25 mM MnCl2. These results indicate that Therminator DNA polymerase can recognize both a TNA primer and tNTP substrates and is an effective catalyst of TNA polymerization despite changes in the geometry of the reactants.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000229244600047
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/19009
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Allen Horhota,Keyong Zou,Justin K. Ichida,et al. Kinetic analysis of an efficient DNA-dependent TNA polymerase[J]. J. Am. Chem. Soc.,2005,127(20):7427-7434.
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