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学科主题: 生命有机化学
题名: Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor
其他题名: 神经元限制性沉默因子397位半胱氨酸在其折叠中发挥重要作用
作者: Zhang Y(张岩) ; Hu W(胡伟) ; Shen J(申杰) ; Tong XT(童孝田) ; Yang ZZ(杨中正) ; Chen ZZ(谌章舟) ; Lan WX(蓝文贤) ; Wu HM(吴厚铭) ; Cao CY(曹春阳)
通讯作者: 曹春阳
刊名: Biochem. Biophys. Res. Commun.
发表日期: 2011
卷: 414, 期:2, 页:309-314
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所
英文摘要: The neuron-restrictive silencer factor/RE1-silencing transcription factor (NRSF/REST) is regarded as not only a key transcriptional repressor but also an activator in neuron gene expression by specifically interacting with neuron-restrictive silencer element (NRSE/RE1) dsDNA and small NRSE/RE1 dsRNA, respectively. But its exact mechanism remains unclear. One major problem is that it is hard to obtain its functional multiple zinc finger (ZnF) domains in a large quantity for further structural studies. To address this issue, in this study, we for the first time attained soluble NRSF/REST functional domains named as ZnF5-8, ZnF4-8, ZnF3-8 and ZnF2-8 containing four, five, six and seven ZnF motifs in tandem, respectively, by using Circular Dichroism (CD) spectrum and two-dimensional (2D) nucleic magnetic resonance (NMR) (1)H-(1)H NOESY spectrum to monitor the folding of each single ZnF peptide. The data indicated that the residue cysteine 397 (Cys397) plays important roles in the global folding of NRSF/REST multiple ZnFs domain. (C) 2011 Elsevier Inc. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000296404800007
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17407
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Zhang Y,Hu W,Shen J,et al. Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor[J]. Biochem. Biophys. Res. Commun.,2011,414(2):309-314.
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