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Solution structure of BmP08, a novel short-chain scorpion toxin from Buthus martensi Karsch
其他题名马氏钳蝎一个新颖短链蝎毒BmP08的溶液结构
Chen X(陈翔); LI YIMING; Tong XT(童孝田); Zhang NX(张乃霞); Wu G(吴宫); ZHANG QI; Wu HM(吴厚铭)
2005
发表期刊Biochem. Biophys. Res. Commun.
ISSN0006-291X
卷号330期号:4页码:1116-1126
摘要A novel short-chain scorpion toxin BmP08 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange, and reversed-phase chromatography. The primary sequence of BmP08 was determined using the tandem MS/MS technique and Edman degradation, as well as results of NMR sequential assignments. It is composed of 31 amino acid residues including six cysteine residues and shares less than 25% sequence identity with the known alpha-KTx toxins. BmP08 shows no inhibitory activity on all tested voltage-dependent and Ca2+-activated potassium channels. The 3D-structure of BmP08 has been determined by 2D-NMR spectroscopy and molecular modeling techniques. This toxin adopts a common alpha/beta-motif, but shows a distinctive local conformation and features a 3(10)-helix and a shorter beta-sheet. The unique structure is closely related to the distinct primary sequence of the toxin, especially to the novel arrangement of S-S linkages in the molecule, in which two disulfide bridges (C-i-C-j and Ci+3-Cj+3) link covalently the 3(10)-helix with one strand of the beta-sheet structure. The electrostatic potential surface analysis of the toxin reveals salt bridges and hydrogen bonds between the basic residues and negatively charged residues nearby in BmP08, which may be unfavorable for its binding with the known voltage-dependent and Ca2+-activated potassium channels. Thus, finding the target for this toxin should be an interesting task in the future. (c) 2005 Elsevier Inc. All rights reserved.
学科领域生命有机化学
部门归属上海有机所
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收录类别SCI
语种英语
WOS记录号WOS:000228566300016
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/17363
专题上海有机化学研究所
通讯作者Wu HM(吴厚铭)
推荐引用方式
GB/T 7714
Chen X,LI YIMING,Tong XT,et al. Solution structure of BmP08, a novel short-chain scorpion toxin from Buthus martensi Karsch[J]. Biochem. Biophys. Res. Commun.,2005,330(4):1116-1126.
APA 陈翔.,LI YIMING.,童孝田.,张乃霞.,吴宫.,...&吴厚铭.(2005).Solution structure of BmP08, a novel short-chain scorpion toxin from Buthus martensi Karsch.Biochem. Biophys. Res. Commun.,330(4),1116-1126.
MLA 陈翔,et al."Solution structure of BmP08, a novel short-chain scorpion toxin from Buthus martensi Karsch".Biochem. Biophys. Res. Commun. 330.4(2005):1116-1126.
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