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学科主题: 生命有机化学
题名: Solution structure of BmKK2, a new potassium channel blocker from the venom of Chinese scorpion Buthus martensi Karsch
其他题名: 中国马氏钳蝎毒液中一种新的钾通道堵塞肽BmKK2的溶液结构
作者: Zhang NX(张乃霞) ; Li MH(李明华) ; Chen X(陈翔) ; Wang YF(王跃丰) ; Wu G(吴宫) ; Hu GY(胡国渊) ; Wu HM(吴厚铭)
通讯作者: 吴厚铭
刊名: Proteins
发表日期: 2004
卷: 55, 期:4, 页:835-845
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室; 中国科学院上海药物所
英文摘要: A natural K+ channel blocker, BmKK2 (a member of scorpion toxin subfamily alpha-KTx 14), which is composed of 31 amino acid residues and purified from the venom of the Chinese scorpion Buthus martensi Karsch, was characterized using whole-cell patch-clamp recording in rat hippocampal neurons. The three dimensional structure of BmKK2 was determined with two-dimensional NMR spectroscopy and molecular modelling techniques. In solution this toxin adopted a common alpha/beta-motif, but showed distinct local conformation in the loop between alpha-helix and beta-sheet in comparison with typical short-chain scorpion toxins (e.g., CTX and NTX). Also, the a helix is shorter and the P-sheet element is smaller (each strand consisted only two residues). The unusual structural feature of BmKK2 was attributed to the shorter loop between the a-helix and P-sheet and the presence of two consecutive Pro residues at position 21 and 22 in the loop. Moreover, two models of BmKK2/hKv1.3 channel and BmKK2/rSK2 channel complexes were simulated with docking calculations. The results demonstrated the existence of a a-mode binding between the toxin and the channels. The model of BmKK rSK2 channel complex exhibited favorable contacts both in electrostatic and hydrophobic, including a network of five hydrogen bonds and bigger interface containing seven pairs of inter-residue interactions. In contrast, the model of BmKK2/hKv1.3 channel complex, containing only three pairs of interresidue interactions, exhibited poor contacts and smaller interface. The results well explained its lower activity towards Kv channel, and predicted that it may prefer a type of SK channel with a narrower entryway as its specific receptor. (C) 2004 Wiley-Liss, Inc.
语种: 英语
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WOS记录号: WOS:000221802000006
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17351
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Zhang NX,Li MH,Chen X,et al. Solution structure of BmKK2, a new potassium channel blocker from the venom of Chinese scorpion Buthus martensi Karsch[J]. Proteins,2004,55(4):835-845.
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