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学科主题: 生命有机化学
题名: Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch
其他题名: 马氏钳蝎毒液中的一种新颖钾通道堵塞肽BmKK2 (alpha-KTx 14.2)的纯化和药理表征
作者: Li MH(李明华) ; Zhang NX(张乃霞) ; Chen XQ(陈学青) ; Wu G(吴宫) ; Wu HM(吴厚铭) ; Hu GY(胡国渊)
通讯作者: 吴厚铭
刊名: Toxicon
发表日期: 2004
卷: 43, 期:8, 页:895-900
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室; 中国科学院上海药物所
英文摘要: BmKK2 (alpha-KTx 14.2) is one of the novel short-chain peptides found in molecular cloning of a venom gland cDNA library from Asian scorpion Buthus martensi Karsch. Based upon its amino acid sequence, the peptide was proposed to adopt a classical alpha/beta-scaffold for alpha-KTxs. In the present study, we purified BmKK2 from the venom of B. martensi Karsch, and investigated its action on voltage-dependent K+ currents in dissociated hippocampal neurons from neonatal rats. BmKK2 (10-100 muM) selectively inhibited the delayed rectifier K+ current, but did not affect the fast transient K+ current. The inhibition of BmKK2 on the delayed rectifier K+ current was reversible and voltage-independent. The peptide did not affect the steady-state activation of the current, but caused a depolarizing shift (about 9 mV) of its steady-state inactivation curve. The results demonstrate that BmKK2 is a novel K+ channel-blocking scorpion peptide. (C) 2004 Elsevier Ltd. All rights reserved.
语种: 英语
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WOS记录号: WOS:000222564500005
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17345
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Li MH,Zhang NX,Chen XQ,et al. Purification and pharmacological characterization of BmKK2 (alpha-KTx 14.2), a novel potassium channel-blocking peptide, from the venom of Asian scorpion Buthus martensi Karsch[J]. Toxicon,2004,43(8):895-900.
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