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学科主题: 天然产物有机化学
题名: Enterococcus durans产胞外多糖EPS-I的分离纯化和结构分析
其他题名: Isolation, purification and structural elucidation of EPS-I, an extracellular polysaccharide from Enterococcus durans
作者: 顾笑梅 ; 吴厚铭 ; 马桂荣
通讯作者: 马桂荣
刊名: 高等学校化学学报
发表日期: 2004-01-01T00:00:00Z
卷: 25, 期:7, 页:1288-1290
收录类别: SCI
部门归属: 山东大学; 中国科学院上海有机化学研究所生命有机化学实验室
英文摘要: The rough crystal of exopolysaccharide produced by Enterococcus durans, a strain of lactic acid bacteria screened from the intestine of a cock in our laboratory, was purified by CM-cellulose column chromatography, DEAE-Sephadex A-25 ionexchange and Sephadex G-100 gel chromatography to give EPS-I. The EPS-I was eluted as a single peak in HPLC analysis, indicating the homogeneity of EPS-I and free from low-molecular-weight polysaccharides. The molecular weight of the EPS-I was determined as 42 000 by the light scattering method. The result of its elemental analysis was C 41.08% and H 7.23% without the elements of N, P and S. Monosaccharide analysis showed that it was composed of Glc and Man in a molar ratio of 4:1. Sugar composition analysis, methylation analysis and H-1 and C-13 NMR spectroscopy revealed that the EPS-I was composed of pentasaccharide repeating units. The sequence of sugar residues was determined by using two-dimensional NMR, including heteronuclear multiple-bond correlation(HMBC) and nuclear overhauser effect spectroscopy (NOESY). The structure of the pentasaccharide repeating unit of EPS-I was given, a new excellular polysaccharide from lactic acid bacterium compared with other EPSs was reported.
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17343
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
顾笑梅,吴厚铭,马桂荣. Enterococcus durans产胞外多糖EPS-I的分离纯化和结构分析[J]. 高等学校化学学报,2004,25(7):1288-1290.
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