SIOC OpenIR  > 上海有机化学研究所
The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H
其他题名细胞色素B5及其突变子V45H溶液结构的比较研究
Zhang Q(张琪); Cao CY(曹春阳); Wang ZQ(王子强); Wang YH(王韵华); Wu HM(吴厚铭); Huang ZX(黄仲贤)
2004
发表期刊Protein Sci.
ISSN0961-8368
卷号13期号:8页码:2161-2169
摘要A comparative study on the solution structures of bovine microsomal cytochrome b(5) (Tb-5) and the mutant V45H has been achieved by 1D and 2D H-1-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by similar to3degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
学科领域天然产物有机化学
部门归属中国科学院上海有机化学研究所生命有机化学实验室; 复旦大学
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000222928800018
引用统计
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/17339
专题上海有机化学研究所
通讯作者Wu HM(吴厚铭); Huang ZX(黄仲贤)
推荐引用方式
GB/T 7714
Zhang Q,Cao CY,Wang ZQ,et al. The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H[J]. Protein Sci.,2004,13(8):2161-2169.
APA 张琪,曹春阳,王子强,王韵华,吴厚铭,&黄仲贤.(2004).The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H.Protein Sci.,13(8),2161-2169.
MLA 张琪,et al."The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H".Protein Sci. 13.8(2004):2161-2169.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2004097.pdf(165KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[张琪]的文章
[曹春阳]的文章
[王子强]的文章
百度学术
百度学术中相似的文章
[张琪]的文章
[曹春阳]的文章
[王子强]的文章
必应学术
必应学术中相似的文章
[张琪]的文章
[曹春阳]的文章
[王子强]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。