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学科主题: 天然产物有机化学
题名: The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H
其他题名: 细胞色素B5及其突变子V45H溶液结构的比较研究
作者: Zhang Q(张琪) ; Cao CY(曹春阳) ; Wang ZQ(王子强) ; Wang YH(王韵华) ; Wu HM(吴厚铭) ; Huang ZX(黄仲贤)
通讯作者: 吴厚铭 ; 黄仲贤
刊名: Protein Sci.
发表日期: 2004
卷: 13, 期:8, 页:2161-2169
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室; 复旦大学
英文摘要: A comparative study on the solution structures of bovine microsomal cytochrome b(5) (Tb-5) and the mutant V45H has been achieved by 1D and 2D H-1-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by similar to3degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000222928800018
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17339
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Zhang Q,Cao CY,Wang ZQ,et al. The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b(5) and mutant V45H[J]. Protein Sci.,2004,13(8):2161-2169.
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