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学科主题: 天然产物有机化学
题名: The solution structure of the oxidized bovine microsomal cytochrom b5 mutant V61H
其他题名: 细胞色素b5突变子V61H的溶液结构
作者: Cao CY(曹春阳) ; Zhang Q(张琪) ; Xue LL(薛林龙) ; Ma JB(麻锦彪) ; Wang YH(王蕴华) ; Wu HM(吴厚铭) ; Huang ZX(黄仲贤)
通讯作者: 吴厚铭 ; 黄仲贤
刊名: Biochem. Biophys. Res. Commun.
发表日期: 2003-01-01
卷: 307, 期:3, 页:600-609
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室; 复旦大学
英文摘要: Using 1488 NOE constraints. 19 stereo-specific assignments. 13 pairs of H-bond constraints, and 140 pseudo-contact shift constraints, a family of 35 structures of bovine microsomal cytochrome bs mutant V61 H has been obtained through the program PSEUDYANA. The family has been further refined by restrained energy minimization to give a family of final stnlctures. The RMSri values of final structures with respect to the average structure are O.45 ±O.11 and 0.96±I O.l0 A for backbone and heavy atoms, respectively. The final △ Xax and △Xrh values are 2.34 x 1O-32 and -O.67 x 1O-32 m3 respectively. The comparisons between the solution structures of mutant V61 H and Wf cytochrome bs. And X-ray stnlcture of the mutant V61H show that the global folding of the molecule in solution is unchanged and the side-chain of His61 deviates from the heme pocket and extends into the solvent like in its crystal structure. However, the helices around the heme pocket undergo outward global displacement while their local con-formations are well maintained. Meanwhile, the heme ring shows a little off the heme pocket, which accounts for the lower stability of the mutant. Additionally, the axial ligand rings counterclockwise rotate around His39 N-Fe axis due to the mutation, which is confirmed by variation of the hyperfine shifts of the heme protons of V61 H compared to those of WT cytochrome b5. _
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17335
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Cao CY,Zhang Q,Xue LL,et al. The solution structure of the oxidized bovine microsomal cytochrom b5 mutant V61H[J]. Biochem. Biophys. Res. Commun.,2003,307(3):600-609.
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