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学科主题: 生命有机化学
题名: Purification, characterization and ~1H NMR resonance assignment of an α-like neurotoxin BmK16 from the vanom of Chinese scorpion buthus martensi karsch
其他题名: 马氏钳蝎毒液中的呔α 类神经毒素BmK16 的纯化, 表征和 1H NMR信号归属
作者: Zhang NX(张乃霞) ; Wu G(吴宫) ; Wang ZH(王中华) ; Wu HM(吴厚铭)
通讯作者: 吴厚铭
刊名: Chin. J. Chem.
发表日期: 2003-01-01
卷: 21, 期:10, 页:1378-1384
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室
英文摘要: A natural scorpion toxin BmK 16 was purified for the first time from the venom of the Chinese scorpion Buthus martensii Karsch (BmK) by using combined gel-filtration. Ion exchange and reversed phase chromatography. The sequence of the N-terminal 8 aminn acid residues was determined by Edman degradation. Using the N-terminal sequence as a tag, the database searching revealed a hit in the scorpion cDNA Bank.The seqaence for N-terminal 8 amino acid residues . Molecular weight and amino acid compositions of BmK 16 were identical with the calculated valaes according to the first 64 residues' se- quence of the precursor peptide alpha-neurotoxin TXI6 derived from the sequence of the cDNA AF156597 (EMBL) . The sequence-specific resonance assignment of BmK 16 was achieved and the 'intact seqaence of BmK 16 was determined as followings: VRDAy IAKPH NCVYE CARNE YCNDL CTKNG AKSGY CQWVG KYGNG CWCKE LPDNV PIRVP GKCH. Furthermore . The results from the sequence homology analysis and the toxicity assays indicated that BmK 16 was an α-like scorpion neurotoxin.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17333
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Zhang NX,Wu G,Wang ZH,et al. Purification, characterization and ~1H NMR resonance assignment of an α-like neurotoxin BmK16 from the vanom of Chinese scorpion buthus martensi karsch[J]. Chin. J. Chem.,2003,21(10):1378-1384.
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