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学科主题: 生命有机化学
题名: 马氏钳蝎蝎毒短肽BmK622的分离纯化和一级结构测定 BmK622 from the venom of chinese scorpion buthus martensii karsch
其他题名: Purification and primary structure of a toxin-like peptide
作者: 张乃霞 ; 吴宫 ; 王跃丰 ; 王中华 ; 冯传莉 ; 吴厚铭
通讯作者: 吴厚铭
刊名: 化学学报
发表日期: 2003-01-01
卷: 61, 期:4, 页:630-634
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室
摘要: 应用凝胶过滤、离子交换和HPLC反相色谱法从马氏钳蝎粗毒中分离纯化得到蝎毒多肽BmK622.联合运用串联质 谱法和Edman降解法,鉴定了BmK622 N 端19个残基的序列,经过数据库检索,发现数据库中用cDNA克隆方法鉴定了序 列的马氏钳蝎蝎毒短肽BmTX3一级序列N端19个残基与BmK622已测定的N 端19个残基序列完全相同,BmK622的分子 量测定和氨基酸组成分析的结果表明,BmK622与BmTx3分子量相同、氨基酸组成一致,从而BmK622的一级结构为: FGLID VKCFA SSECW TACKK VTGSG QGKCQ NNQCR CY.
英文摘要: The toxin-like peptide BmK622 has been purified fmm the venom of the Chinese scorpion Buthus martensii Karsch by using combined gel-filtration , ion exchange and revered phase chromatography techniques . The sequence of the peptide BmK622 was determined by the hybrid applying of tandem CID MS/MS, Edman degradati on and database searching . Firstly the sequence for the N-terminal 19 amino acids of BmK622 was obtained by using tandem CID MS/MS and Edman degradation . The following database searching revealed a hit . The sequence of N-temrnal 19 amino acid residues of BmK622 is identical with that of the scorpion toxin BmTX3 , which was determined by cDNA cloning. In addition, the molecular weight and the amino acid compositions of BmK622 arn consistent with the calculated values according to the sequence of BmTX3 . All these data suggested that BmK622 shared the same primary structure with BmTX3 , and the intact sequence of BmK622 is as follows: FGLID VKCFA SSECW TACKK VTGSG QGKCQ NNQCR CY.
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17331
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
张乃霞,吴宫,王跃丰,等. 马氏钳蝎蝎毒短肽BmK622的分离纯化和一级结构测定 BmK622 from the venom of chinese scorpion buthus martensii karsch[J]. 化学学报,2003,61(4):630-634.
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