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学科主题: 天然产物有机化学
题名: A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol
其他题名: 从云南紫杉提取物中大量分离紫杉烷和7表,10去乙酰紫杉醇的1H-and 13C NMR 归属
作者: Xue J(薛军) ; Cao CY(曹春阳) ; Chen JM(陈建民) ; Bu HS(卜海山) ; Wu HM(吴厚铭)
通讯作者: 陈建民 ; 吴厚铭
刊名: Chin. J. Chem.
发表日期: 2001-01-01
卷: 19, 期:1, 页:82-90
收录类别: SCI
部门归属: 复旦大学; 中国科学院上海有机化学研究所生命有机国家开放实验室
英文摘要: A large-scale separation of paclitaxel from semi-purified bark extract of taxus yunnanesis was investigated. The chromatographic behavior of paclitaxel and two close eluting analogues, cephalomannine and 7-epi-10-deacetyltaxol were systematically studied on a C^18 bonded phase column with different mobile phase in reverse phase mode. According to the notably different selectivity of the methand and acetonitrile with water in the mobile phase and the most important requirement of capacity in preparative chromatography, the optimum suitablymobile phase used in a large-scale isolation of paclitaxel and 7-epi-10-deacetyltaxol on a preparative C^18 column was give. Cephalomannine was eliminated by ozonolysis and after then separated throughout a normal phase silica column. The whole large-scale process for high purity paclitaxel from the bark extract of Taxus yunnanesis consisted of a preliminary purification with biotage FLASH 150i system based on a prepaked normal phase silica cartridge followed by using a C^18 Nova-pak~TM 4000 preparative HPLC system. The structure of 7-epi-10-deacetyltaxol was elucidated by 2D NMR tecnologies of TOCSY, DQF-COSY, HMQC and HMBC etc.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17285
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Xue J,Cao CY,Chen JM,et al. A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol[J]. Chin. J. Chem.,2001,19(1):82-90.
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