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学科主题: 天然产物有机化学
题名: Structural studies of the glycopetides of B-chain of chinnamomin-a type II ribosome-inactivating protein by nuclear magnetic resonance
其他题名: 香樟子糖蛋白B链糖肽的结构研究
作者: Zheng Pu ; Li YM(李医明) ; He FH(何发虎) ; Zhang NX(张乃霞) ; Wu HM(吴厚铭) ; Liu WY(刘望夷)
通讯作者: 吴厚铭 ; 刘望夷
刊名: Glycoconjugate J.
发表日期: 2000-01-01
卷: 17, 期:11, 页:749-759
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室; 上海生化所
英文摘要: Cinnamomin is a pant type II ribosome-inactivating protein (RIP) isolated from the seeds of Cinnamomum camphora. It consists of two nonidentical polypeoptide chains (A-and B-chain) held together through one disulfide linkage its A-and B-chain contain 0.3% and 309% sugars resopectively. The B-chain of cinnamomin was digested by proase E and then the liberated glycopeptides were separated from non-glycopetides by gel filtration chromatography on a Bio-Gel P-4 column. Three crude glycopetides were obtained by continuing chromatography over anion-exchange resin (AG1-X2) in the buffer of 2% pyridine-acetic acid (Ph 8.3) with a polygradient elution system. Through further purification by the gel filtration chromaogrphy and HPLC, three major glycopeptides, GP1, GP2 and GP3 were obtained. Mainly by two-dimenjsional Nuclear Magnetic Resonance (NMR) including TOCSY, DQF-COSY, NOESY, HMQC and HMBC, their primary structures were analyzed as Manα1,3 Manα1,6(Manα1,3)(Xylβ1,2)Manβ1,4GlcNAcβ1,4GlcNAcβ1-(Gly-)Asn-Asn-Thr(GP1), Manα1,6(Manα1,3)(Xylβ1,2)Manβ1,4GlcNAcβ1,4GlcNAcβ1-GlcNAcβ1,4(Fucα1,3)GlcNAcβ1-Asn-Ala-Thr(GP2),Manα1,3)Manα,1,6(Manα1,2 Manα1,3)Manβ, 4GlcNAβ1,4GlcNAcβ1-(-Ala-) Asn-Gly-Thr(GP3).
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17277
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Zheng Pu,Li YM,He FH,et al. Structural studies of the glycopetides of B-chain of chinnamomin-a type II ribosome-inactivating protein by nuclear magnetic resonance[J]. Glycoconjugate J.,2000,17(11):749-759.
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