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学科主题: 天然产物有机化学
题名: 马氏钳蝎毒素中多肽BmK4112的分离纯化和一级结构测定
其他题名: Purification and primary structure of a novel peptide from the Chinese scorpion buthus martensi karsch
作者: 李医明 ; 吴宫 ; 张乃霞 ; 吴厚铭
通讯作者: 吴厚铭
刊名: 生物化学与生物物理学报
发表日期: 2000-01-01
卷: 32, 期:4, 页:351-355
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室
摘要: 对马氏钳竭粗毒中多肽进行分离纯化及鉴定。应用凝胶过滤、离子交换和肥比反相色谱方法分离纯化样品。应用二琉基苏糖醇(DTT)还原,碘乙酸保护法对纯化样品进行修饰,修饰样品经酶解后应用 Edman降解法,并结合ESI—MS法和串联质谱法(A4S—MS)分析肽的序列。比较修饰前后的质谱,测定分子中的二硫键数目。分离纯化获得多肽Bmlt4112,其一级结构为了PYPVNCKTDRD(:VM CGlGISCItNGYCTGQC。分子中含三对二硫键。经搜寻蛋白质序列库, BmK4112为一新的多肤。
英文摘要: Abstract To purify and characterize peptides from the venom of Chinese scorpion Buthus martensi Karsch, the purification was carried out by gel-filtration, ion exchange and reversed phase HPLC techniques. The puri-fied peptide was reduced by dithioerythritol (OTT), S-alkytated with iodoacetic acid, and subjected to enzymat-ic cleavages (TPCK-trypsin). The purified fragments from enzymatic cleavage of the peptide were separated by C18 HPLC, then submitted to the ESL-MS, and Edman degradation for amino acid sequence determination. The mixture was also subjected to tandem mass (MS-MS) analysis. As a result, a novel peptide, named HmK4112, was obtained, with the primary structure being: TPYPV NCIKTD RDCVM CGLGI SCKNG YCTGQ C, and having three disulfide bonds.
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17271
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
李医明,吴宫,张乃霞,等. 马氏钳蝎毒素中多肽BmK4112的分离纯化和一级结构测定[J]. 生物化学与生物物理学报,2000,32(4):351-355.
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