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学科主题: 天然产物有机化学
题名: Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance
其他题名: 香樟子蛋白A链的腺嘌啉核苷N-糖苷酶活性的核磁共振表征
作者: Xu YZ(徐永镇) ; Li YJ(李泳江) ; Hu HY(胡红雨) ; Wu HM(吴厚铭) ; Liu WY(刘望夷)
通讯作者: 刘望夷
刊名: Biol. Chem.
发表日期: 2000-01-01
卷: 381, 期:5-6, 页:447-451
收录类别: SCI
部门归属: 中国科学院上海生华所; 中国科学院上海有机化学研究所生命有机国家开放实验室
英文摘要: plantribosome-inactivating proteins specifically cleave an N-glyoosidic bond of a unique adenosine in The largest ribosomal RNA, releasing an adenine from ribosomes of differentsources. Here, ~1H-nuclear magnetic resonance is useed to analyze the enzymatic products of the A-chain of cinnamomin. A type-II ribosome-inactvating protein (RIP) actng on the nucleotides insitu. The enzymatic of the RIP on nine nuclootides are compared. Cinnamomin A-chain can cleave the N-glycosidic bond and release an adenine base from adenine nucleotides except 5'-ATP; however it cannot act on 5'-GMP, 5'-CMP, and 5' -UMP The A-chain in the mixture of cinnamomin A- and B-chain exhibits htgher activity toward adenine nucleotides than the A-chain alone does, suggesting that the B-chain canformationally stabilize the A-chain. Intact cinnamomin also exhibits lower activity toward adenine nucleotide, However, cinnamomin B-chain and heat-denatured intact cinnamomin cannot hydrolyze all the tested nucleotides. We conclude That hydrolysis of the N-C glycosidic bond of nucleotide compounds by cinnamomin A-chain has a base preference. And the negatively charged phosphate group(s) reduces the recognition ability of the A-chain to adenine nucleotide.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/17267
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Xu YZ,Li YJ,Hu HY,et al. Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance[J]. Biol. Chem.,2000,381(5-6):447-451.
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