Enzymatic transformation of ginsenoside Rg(3) to Rh-2 using newly isolated Fusarium proliferatum ECU2042 Fusarium proliferatum
其他题名一种水果种仁粗粉催化的葡萄糖和醇合成相应b-葡萄糖苷的简便方法
Xu JH(许建和); Lu WY(卢文芽); Lin GQ(林国强)
2006
发表期刊J. Mol. Catal. B-Enzym.
ISSN1381-1177
卷号38期号:2页码:113-118
摘要A new soil isolate, Fusarium proliferatum ECU2042, was found capable of producing a ginsenoside Rg(3) hydrolase (a special beta-glucosidase) which is useful for production of a very potent antitumor agent Rh-2. By investigating its fermentation progress, the optimal time for beta-glucosidase biosynthesis was determined as 72 h. The crude beta-glucosidase was partially purified by 49.4-fold from the cell-free extract with an activity yield of 13%. The catalytic performance of the partially purified beta-glucosidase was examined, giving optima of pH and temperature at pH 5.0-6.0 and 50 degrees C. It was found that the F. proliferatum beta-glucosidase can also hydrolyze cellobiose, but not active towards a frequently used artificial substrate p-nitrophenyl beta-D-alucopyranoside (pNPG). In a lab-scale preparation, the bio-hydrolytic reaction was carried out at 50 degrees C for 24 h in 50 ml NaAc/HAc buffer (100 mM, pH 5.0) containing 50 mg Rg(3) and 12 mg lyophilized crude enzyme extract, giving the ginsenoside Rh-2 in 60.3% conversion. (c) 2005 Elsevier B.V. All rights reserved.
学科领域天然产物有机化学
部门归属华东理工大学; 中国科学院上海有机化学研究所
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收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/16659
专题中科院天然产物有机化学重点实验室
通讯作者Xu JH(许建和); Lin GQ(林国强)
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Xu JH,Lu WY,Lin GQ. Enzymatic transformation of ginsenoside Rg(3) to Rh-2 using newly isolated Fusarium proliferatum ECU2042 Fusarium proliferatum[J]. J. Mol. Catal. B-Enzym.,2006,38(2):113-118.
APA 许建和,卢文芽,&林国强.(2006).Enzymatic transformation of ginsenoside Rg(3) to Rh-2 using newly isolated Fusarium proliferatum ECU2042 Fusarium proliferatum.J. Mol. Catal. B-Enzym.,38(2),113-118.
MLA 许建和,et al."Enzymatic transformation of ginsenoside Rg(3) to Rh-2 using newly isolated Fusarium proliferatum ECU2042 Fusarium proliferatum".J. Mol. Catal. B-Enzym. 38.2(2006):113-118.
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