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A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network
其他题名一种新的由聚(N-丙烯酰三羟甲基氨基甲烷)和聚乙烯吡咯烷酮形成的准互穿网络:用于毛细管电泳紫外检测条件下单链和双链DNA的分离介质
Wang Q(王前); Xu X(许旭); Dai LX(戴立信)
2006
发表期刊Electrophoresis
ISSN0173-0835
卷号27期号:9页码:1749-1757
摘要The preparation of a new separation matrix, quasi-interpenetrating networks (quasi-IPNs) formed by poly(N-acryloyl-Tris) (poly(tris-A)) and PVP, and its application for dsDNA and ssDNA fragments separation by CE with UV detection, are presented. This new quasi-IPN exhibited high sieving performance, good dynamic coating ability, and low viscosity. Single-base resolutions of dsDNA fragments (R-s = 0.92 for 123/124 bp) and ssDNA fragments (R-s = 0.65 for 123/124 base, R-s = 0.48 for 309/310 base) were achieved by using the quasi-IPN of poly(tris-A)/PVP (2% + 2%) solution in a 31 cm effective length linear polyacrylamide (LPA)-coated column. Single-base separation of dsDNA fragments (R,, = 0.92 for 123/124 bp) was also obtained within 28 min in a 46.7 cm effective length bare column at higher 160 V/cm electric field strength by using the same quasi-IPN solution. The RSD of the migration time measured for each DNA fragments was less than 1.5% in the bare column for nine continuous runs. The effects of temperature and electric field strength on the DNA separation were also investigated.
学科领域分析化学
部门归属中国科学院上海有机化学研究所
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收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/15933
专题金属有机化学国家重点实验室
通讯作者Xu X(许旭)
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GB/T 7714
Wang Q,Xu X,Dai LX. A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network[J]. Electrophoresis,2006,27(9):1749-1757.
APA 王前,许旭,&戴立信.(2006).A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network.Electrophoresis,27(9),1749-1757.
MLA 王前,et al."A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network".Electrophoresis 27.9(2006):1749-1757.
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