中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 金属有机化学国家重点实验室  > 期刊论文
学科主题: 分析化学
题名: A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network
其他题名: 一种新的由聚(N-丙烯酰三羟甲基氨基甲烷)和聚乙烯吡咯烷酮形成的准互穿网络:用于毛细管电泳紫外检测条件下单链和双链DNA的分离介质
作者: Wang Q(王前) ; Xu X(许旭) ; Dai LX(戴立信)
通讯作者: 许旭
刊名: Electrophoresis
发表日期: 2006-01-01
卷: 27, 期:9, 页:1749-1757
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所
英文摘要: The preparation of a new separation matrix, quasi-interpenetrating networks (quasi-IPNs) formed by poly(N-acryloyl-Tris) (poly(tris-A)) and PVP, and its application for dsDNA and ssDNA fragments separation by CE with UV detection, are presented. This new quasi-IPN exhibited high sieving performance, good dynamic coating ability, and low viscosity. Single-base resolutions of dsDNA fragments (R-s = 0.92 for 123/124 bp) and ssDNA fragments (R-s = 0.65 for 123/124 base, R-s = 0.48 for 309/310 base) were achieved by using the quasi-IPN of poly(tris-A)/PVP (2% + 2%) solution in a 31 cm effective length linear polyacrylamide (LPA)-coated column. Single-base separation of dsDNA fragments (R,, = 0.92 for 123/124 bp) was also obtained within 28 min in a 46.7 cm effective length bare column at higher 160 V/cm electric field strength by using the same quasi-IPN solution. The RSD of the migration time measured for each DNA fragments was less than 1.5% in the bare column for nine continuous runs. The effects of temperature and electric field strength on the DNA separation were also investigated.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/15933
Appears in Collections:金属有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2006206.pdf(241KB)----开放获取--View Download

Recommended Citation:
Wang Q,Xu X,Dai LX. A new quasi-interpenetrating network formed by poly(N-acryloyl-tris(hydroxymethyl)aminomethane and polyvinylpyrrolidone: Separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection poly(N-acryloyl-tris(hydroxymethyl)aminomethane; polyvinylpyrroliclone; quasi-interpenetrating network[J]. Electrophoresis,2006,27(9):1749-1757.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[王前]'s Articles
[许旭]'s Articles
[戴立信]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[王前]‘s Articles
[许旭]‘s Articles
[戴立信]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2006206.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace