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学科主题: 分析化学
题名: Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography AND LIFE SCIENCES rhein; berberine
其他题名: 柱切换高效液相色谱法同时测定大鼠血浆中的黄芩苷、大黄酸和小檗碱
作者: Li Y(李奕) ; Gao JP(高建平) ; Xu X(许旭) ; Dai LX(戴立信)
通讯作者: 许旭
刊名: J. Chromatogr. B
发表日期: 2006-01-01T00:00:00Z
卷: 838, 期:1, 页:50-55
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所; 上海中医药大学中药学院
英文摘要: A simple HPLC method using column-switching and ultraviolet detection was developed for the simultaneous determination of baicalin (BA), rhein (RH) and berberine (BE) in rat plasma. Plasma samples were injected directly onto a Capcell Pak MF C-8 column (150 mm x 4.6 mm i.d.) to remove protein and to be pre-separated by an isocratic elution using 50 mmol/L phosphate sodium (pH 6.85)-acetonitrile (10:1, v/v). After the drug-containing fractions were transferred to a Kromasil C column (150 nun x 4.6 turn i.d.) by a valve switching step, the valve position was switched back and the main separation was performed by an isocratic elution using triethylamine adjusted 20 mmoL/L phosphoric acid (pH 6.78)-acetonitrile (4: 1, v/v). The flow rate was always 1.0 mL/min. The calibration curve showed excellent linear relationship (r >= 0.9997) over the concentration range of 0.4-7.9 mu g/mL for baicalin, 0.2-7.8 mu g/mL for rhein and 0.4-7.7 mu g/mL for berberine in rat plasma. The intra- and inter-day assay precisions (R.S.D.) of three analytes were in the range of 0.34-4.3% and the accuracies were between 98.0% and 102.4%. Their recoveries were all greater than 95%. The method was successfully applied to the multi-constituents plasma concentration-time curve study after oral administration of a traditional Chinese medicine prescription Xiexin-Tang in rats. (c) 2006 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/15929
Appears in Collections:金属有机化学国家重点实验室_期刊论文

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Recommended Citation:
Li Y,Gao JP,Xu X,et al. Simultaneous determination of baicalin, rhein and berberine in rat plasma by column-switching high-performance liquid chromatography AND LIFE SCIENCES rhein; berberine[J]. J. Chromatogr. B,2006,838(1):50-55.
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